APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
0.25-0.5μg/ml for 30 minutes at RT |
1-2μg/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human NEFL protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CMT1F , CMT2E , NEFL , NF68 , NFL , PPP1R110 , neurofilament, light polypeptide , NF-L , NF-68 , neurofilament light , 68kDa Neurofilament Light
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
|
GTX34904 IHC-P Image
IHC-P analysis of rat cerebellum tissue using GTX34904 NF-L antibody [NFL/736].
|
|
GTX34904 FACS Image
FACS analysis of HEK293 cells using GTX34904 NF-L antibody [NFL/736]. Blue : Primary antibody Red : Isotype control
|
REFERENCE
There are currently no references for NF-L antibody [NFL/736] (GTX34904). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NF-L antibody [NFL/736] (GTX34904). Be the first to share your experience with this product.