GeneTex
United States (US)

NF-L antibody [NR-4]

GTX34902_20170712_IHC-P_18121410_157.jpg
GTX34902_20170712_IHC-P_1_18121410_843.jpg

Cat No. GTX34902

Host Mouse
Clonality Monoclonal
Clone Name NR-4
Isotype IgG1
Application IHC-P
Reactivity Human, Rat, Bovine, Chicken, Pig
See all NF-L products
APPLICATION

Application Note

IHC-P:0.25-0.5ug/ml for 30 minutes at RT.

Calculated MW

62 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Crude neurofilament preparation from porcine spinal cord

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofilament Light,Cmt1F,Cmt2E,Cmtdig,Nf-L,Nf68,Nfl,Ppp1R110,Nefl

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
GTX34902_20170712_IHC-P_18121410_157.jpg

GTX34902 IHC-P Image

Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).
GTX34902_20170712_IHC-P_1_18121410_843.jpg

GTX34902 IHC-P Image

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).
Package List Price ($)
$ 329