GeneTex
United States (US)

NF-L antibody

GTX101142_40737_20170503_IFA_R_19011506_560.jpg
GTX101142_40737_20170531_IHC-Fr_M_19011506_278.jpg
GTX101142_40737_WB_19011506_954.jpg
GTX101142_40737_20170615_WB_M_cerebellum_19011506_301.jpg
GTX101142_40737_IHC_19011506_732.jpg
GTX101142_40737_20170706_WB_H_brain_19011506_989.jpg

Cat No. GTX101142

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

human brain , PC-3 , U87-MG , SK-N-SH , mouse cerebellum

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NF-L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofilament Light,Cmt1F,Cmt2E,Cmtdig,Nf-L,Nf68,Nfl,Ppp1R110,Nefl

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101142_40737_20170503_IFA_R_19011506_560.jpg

GTX101142 ICC/IF Image

NF-L antibody detects NF-L protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-L protein stained by NF-L antibody (GTX101142) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101142_40737_20170531_IHC-Fr_M_19011506_278.jpg

GTX101142 IHC-Fr Image

NF-L antibody detects NF-L protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-L protein stained by NF-L antibody (GTX101142) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101142_40737_WB_19011506_954.jpg

GTX101142 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
B: U87-MG
C: SK-N-SH
7.5% SDS PAGE
GTX101142 diluted at 1:10000

GTX101142_40737_20170615_WB_M_cerebellum_19011506_301.jpg

GTX101142 WB Image

Mouse tissue extract (5 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX101142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101142_40737_IHC_19011506_732.jpg

GTX101142 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using 68kDa Neurofilament Light(GTX101142) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101142_40737_20170706_WB_H_brain_19011506_989.jpg

GTX101142 WB Image

Human brain (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX101142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169