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NF-L antibody

Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101142
Anti-NF-L antibody used in IHC (Frozen sections) (IHC-Fr). GTX101142
Anti-NF-L antibody used in Western Blot (WB). GTX101142
Anti-NF-L antibody used in Western Blot (WB). GTX101142
Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX101142
Anti-NF-L antibody used in Western Blot (WB). GTX101142
Anti-NF-L antibody used in ELISA (ELISA). GTX101142
Anti-NF-L antibody used in ELISA (ELISA). GTX101142

Cat No. GTX101142

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, ELISA, sELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture: GTX101142 , Detection: GTX24572

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

human brain , PC-3 , U87-MG , SK-N-SH , mouse cerebellum

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NF-L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurofilament light , CMT1F , CMT2E , CMTDIG , NF-L , NF68 , NFL , PPP1R110

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101142

GTX101142 ICC/IF Image

NF-L antibody detects NF-L protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-L protein stained by NF-L antibody (GTX101142) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-L antibody used in IHC (Frozen sections) (IHC-Fr). GTX101142

GTX101142 IHC-Fr Image

NF-L antibody detects NF-L protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-L protein stained by NF-L antibody (GTX101142) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-L antibody used in Western Blot (WB). GTX101142

GTX101142 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
B: U87-MG
C: SK-N-SH
7.5% SDS PAGE
GTX101142 diluted at 1:10000

Anti-NF-L antibody used in Western Blot (WB). GTX101142

GTX101142 WB Image

Mouse tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX101142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX101142

GTX101142 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using 68kDa Neurofilament Light(GTX101142) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NF-L antibody used in Western Blot (WB). GTX101142

GTX101142 WB Image

Human brain (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX101142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NF-L antibody used in ELISA (ELISA). GTX101142

GTX101142 ELISA Image

Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX24572 as detection antibody was diluted at 1:1000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-NF-L antibody used in ELISA (ELISA). GTX101142

GTX101142 ELISA Image

Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX60544 as detection antibody was diluted at 1:10000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169