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NF-L antibody

Anti-NF-L antibody used in Western Blot (WB). GTX30190
Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30190

Cat. No. GTX30190

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine, Pig, Avian
Package
50 μl ($739)
See all NF-L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:20000
ICC/IF 1:500 - 1:5000
IHC-P 1:5000
Not tested in other applications.

Product Note

Specifically recognizes the light neurofilament subunit (~68 kDa).

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament light , NF-L

Database

Research Area

DATA IMAGES

Anti-NF-L antibody used in Western Blot (WB). GTX30190

GTX30190 WB Image

WB analysis of rat cerebellum tissue using GTX30190 NF-L antibody.
Dilution : 1:20000

Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30190

GTX30190 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures from newborn rat brain using GTX30190 NF-L antibody (red), co-stained with anti-peripherin antibody (green) and DAPI . A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image.

REFERENCE

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Package List Price ($)
$ 739