GeneTex
United States (US)

NF-L antibody

GTX30190_185_ICC-IF_18121410_514.jpg
GTX30190_1356_WB_18121410_116.jpg

Cat No. GTX30190

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Bovine, Pig, Avian
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:20000
ICC/IF 1:500 - 1:5000
IHC-P 1:5000
Not tested in other applications.

Specificity/Sensitivity

Specifically recognizes the light neurofilament subunit (~68 kDa).
PROPERTIES

Form

Liquid

Buffer

Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX30190_185_ICC-IF_18121410_514.jpg

GTX30190 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures from newborn rat brain using GTX30190 NF-L antibody (red), co-stained with anti-peripherin antibody (green) and DAPI . A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image.

GTX30190_1356_WB_18121410_116.jpg

GTX30190 WB Image

WB analysis of rat cerebellum tissue using GTX30190 NF-L antibody.
Dilution : 1:20000

Package List Price ($)
$ 329