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NF-L antibody

Anti-NF-L antibody used in Western Blot (WB). GTX32419
Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32419
Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX32419
Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX32419
Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX32419

Cat No. GTX32419

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($329)
See all NF-L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-543 of human NEFL (NP_006149.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurofilament light , CMT1F , CMT2E , CMTDIG , NF-L , NF68 , NFL , PPP1R110

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-L antibody used in Western Blot (WB). GTX32419

GTX32419 WB Image

WB analysis of MCF-7 cell lysate using GTX32419 NF-L antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32419

GTX32419 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32419 NF-L antibody.
Blue : DAPI

Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX32419

GTX32419 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32419 NF-L antibody.
Dilution : 1:200

Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX32419

GTX32419 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32419 NF-L antibody.
Dilution : 1:200

Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX32419

GTX32419 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32419 NF-L antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 329