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NF-M antibody, Internal

Anti-NF-M antibody, Internal used in Western Blot (WB). GTX88324

Cat. No. GTX88324

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μg ($419)
See all NF-M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.03-0.1μg/ml
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88324-PEP).

Product Note

This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.

Predict Reactivity

Human, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament medium , NEF3 , NF-M , NFM

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-M antibody, Internal used in Western Blot (WB). GTX88324

GTX88324 WB Image

WB analysis of mouse brain lysate using GTX88324 NF-M antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419