GeneTex
United States (US)

NF-M antibody, Internal

GTX88324_2418_WB_18121411_241.jpg

Cat No. GTX88324

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88324-PEP).

Specificity/Sensitivity

This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.

Predict Reactivity

Human, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofilament Medium,Nef3,Nf-M,Nfm,Nefm

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
GTX88324_2418_WB_18121411_241.jpg

GTX88324 WB Image

WB analysis of mouse brain lysate using GTX88324 NF-M antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329