GeneTex
United States (US)

NF-M antibody [NF-09]

160kDa-Neurofilament-medium-antibody-NF-09-GTX27794-ICC_IF-1_18121409_422.jpg
160kDa-Neurofilament-medium-antibody-NF-09-GTX27794-WB-1_18121409_204.jpg
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Cat No. GTX27794

Host Mouse
Clonality Monoclonal
Clone Name NF-09
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, ELISA
Reactivity Human, Mouse, Rat, Bovine, Pig
APPLICATION

Application Note

ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

102 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Pellet of pig brain cold stable proteins after depolymerization of microtubules.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P07197, NEFM, 4741, NF-M, NEF3, 162250, 160kDa Neurofilament medium, NFM, NF M

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H.

Database

Research Area

DATA IMAGES
160kDa-Neurofilament-medium-antibody-NF-09-GTX27794-ICC_IF-1_18121409_422.jpg

GTX27794 ICC/IF Image

Immunofluorescence staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09 conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).

160kDa-Neurofilament-medium-antibody-NF-09-GTX27794-WB-1_18121409_204.jpg

GTX27794 WB Image

Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

img6501_18121411_195.jpg

GTX27794 Image


Package List Price ($)
$ 319