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NF-M antibody

Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215
Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133215
Anti-NF-M antibody used in Western Blot (WB). GTX133215

Cat No. GTX133215

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NF-M. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neurofilament, medium polypeptide,NF-M,NF160,NF165,Nef3,Nfm

Background

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Database

Research Area

DATA IMAGES
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

GTX133215 IHC-P Image

NF-M antibody detects NF-M protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NF-M antibody (GTX133215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

GTX133215 IHC-P Image

NF-M antibody detects NF-M protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NF-M antibody (GTX133215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133215

GTX133215 IHC-Fr Image

NF-M antibody detects NF-M protein expression by immunohistochemical analysis.
Sample:Frozen-sectioned adult mouse cerebellum.
Green: NF-M protein stained by NF-M antibody (GTX133215) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-M antibody used in Western Blot (WB). GTX133215

GTX133215 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody (GTX133215) diluted at 1:10000.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169