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NF-M antibody

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133215
Anti-NF-M antibody used in Western Blot (WB). GTX133215
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215
Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

Cat No. GTX133215

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NF-M. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886873

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurofilament, medium polypeptide , NF-M , NF160 , NF165 , Nef3 , Nfm

Background

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Database

Research Area

DATA IMAGES

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133215

GTX133215 IHC-Fr Image

NF-M antibody detects NF-M protein expression by immunohistochemical analysis.
Sample:Frozen-sectioned adult mouse cerebellum.
Green: NF-M protein stained by NF-M antibody (GTX133215) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-M antibody used in Western Blot (WB). GTX133215

GTX133215 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody (GTX133215) diluted at 1:10000.

Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

GTX133215 IHC-P Image

NF-M antibody detects NF-M protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NF-M antibody (GTX133215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-M antibody used in IHC (Paraffin sections) (IHC-P). GTX133215

GTX133215 IHC-P Image

NF-M antibody detects NF-M protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NF-M antibody (GTX133215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

NF-M antibody Cat No. GTX133215

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Acta Neuropathol Commun in 2019.PMID: 30626447
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169