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NF-M antibody

Anti-NF-M antibody used in Western Blot (WB). GTX133218
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133218
Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133218
Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133218

Cat No. GTX133218

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse NF-M. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neurofilament, medium polypeptide,NF-M,NF160,NF165,Nef3,Nfm

Background

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Database

Research Area

DATA IMAGES
Anti-NF-M antibody used in Western Blot (WB). GTX133218

GTX133218 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody (GTX133218) diluted at 1:1000.

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133218

GTX133218 ICC/IF Image

NF-M antibody detects NF-M protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-M protein stained byNF-M antibody (GTX133218) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133218

GTX133218 IHC-Fr Image

NF-M antibody detects NF-M protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: NF-M protein stained by NF-M antibody (GTX133218) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX133218

GTX133218 IHC-Fr Image

NF-M antibody detects NF-M protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-M protein stained by NF-M antibody (GTX133218) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169