GeneTex
United States (US)

NF-M antibody

GTX30441_209_ICC-IF_18121410_137.jpg
GTX30441_1401_WB_18121410_570.jpg
GTX30441_360_IHC-Fr_18121410_493.jpg

Cat No. GTX30441

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Bovine, Cat, Pig, Avian
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000 - 1:10000
ICC/IF 1:500 - 1:1000
IHC-P 1:1000 - 1:5000
IHC-Fr 1:1000 - 1:5000
Not tested in other applications.

Specificity/Sensitivity

Specifically recognizes the evolutionarily conserved extreme C-terminal region of Neurofilament Medium (~145-170kDa).
PROPERTIES

Form

Liquid

Buffer

Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat Neurofilament Medium fusion protein corresponding to the C-terminus [UniProt# P12839]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX30441_209_ICC-IF_18121410_137.jpg

GTX30441 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30441 NF-M antibody.
Red : NF-M antibody
Green : Fox1 antibody
Blue : DAPI

GTX30441_1401_WB_18121410_570.jpg

GTX30441 WB Image

WB analysis of rat cerebellum tissue using GTX30441 NF-M antibody.

GTX30441_360_IHC-Fr_18121410_493.jpg

GTX30441 IHC-Fr Image

IHC-Fr analysis of rat cerebral cortex tissue using GTX30441 NF-M antibody. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons.
Red : primary antibody
Green : beta-adrendergic receptor kinase 1
Dilution : 1:5000

Package List Price ($)
$ 319