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NF-M antibody

Orthogonal
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30667
Anti-NF-M antibody used in Western Blot (WB). GTX30667

Cat. No. GTX30667

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Cat, Chicken, Fish
Package
250 μl ($609)
See all NF-M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000
ICC/IF 1:2000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

10mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

The C-terminal extension of rat Neurofilament Medium protein (the so-called KE segment) [UniProt# P12839]

Purification

Ammonium sulfate precipitation

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament medium , Nef3 , Nfm

Database

Research Area

DATA IMAGES

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30667

GTX30667 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30667 NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.

Anti-NF-M antibody used in Western Blot (WB). GTX30667

GTX30667 WB Image

Orthogonal

WB analysis of SH-SY5Y cell lysate using GTX30667 NF-M antibody. ~100kDa band is seen in addition to the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
Lane A : SH-SY5Y cell
Lane B : SH-SY5Y treated with maitotoxin

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 609