GeneTex

  • Country / Location Selection

United States (US)

NF-M antibody

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

Cat. No. GTX85461

Reference 3

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all NF-M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC 1:1000-1:2000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NFM protein was purified from bovine spinal cords following the method of Delacourte et al.

Purification

IgY fraction

Conjugation

Unconjugated

RRID

AB_10629221

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament medium , NEF3 , NF-M , NFM

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250.
Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

ICC/IF analysis of mouse cortical neurons using GTX85461 NF-M antibody.
Green : Primary antibody
Red : GFAP
Blue : DAPI
Dilution : 1:2000
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

Dissociated cell culture prepared from an adult rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to co-stain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

The data was published in the journal PLoS Biol in 2015.PMID: 26406915

REFERENCE

Application Reference

Brugger V et al. Nat Commun 2017; 8 : 14272 Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.
Brugger V et al. PLoS Biol 2015; 13 (9) : e1002258 HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.
Application : ICC/IF
Reactivity : Mouse
Pelayo JC et al. Cell Tissue Res 2014; Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for NF-M antibody (GTX85461). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399