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NF-M antibody

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

Cat No. GTX85461

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat
Package
50 μg ($289)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC 1:1000-1:2000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 1%BSA, sodium azide (0.02%, w/v)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NFM protein was purified from bovine spinal cords following the method of Delacourte et al.

Purification

IgY fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofilament Medium,Nef3,Nf-M,Nfm,Nefm

Cellular Localization

Cytoplasmic

Background

Human Neurofilament Medium Chain (NF-M) is a 102,448 dalton protein (916 amino acids) expressed in CNS and PNS neurons. This cytoskeletal protein is normally phosphorylated in vivo, and its extent of phosphorylation correlates with different states of axonal growth and stabilization. These observations have led to the hypothesis that the NF-M protein cross-links adjacent neurofilament strands, and contributes to the structural integrity of the axon.

Database

DATA IMAGES
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250.
Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

ICC/IF analysis of mouse cortical neurons using GTX85461 NF-M antibody.
Green : Primary antibody
Red : GFAP
Blue : DAPI
Dilution : 1:2000

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85461

GTX85461 ICC/IF Image

Dissociated cell culture prepared from an adult rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to co-stain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining

REFERENCE
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Package List Price ($)
$ 289