GeneTex
United States (US)

NF-M antibody

NF-M-antibody-GTX85461-IHC-1_18122102_212.jpg
GTX85461__20161004_IFA_18122102_136.jpg
NF-M-antibody__GTX85461-1_18122101_720.jpg

Cat No. GTX85461

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Recommended starting dilutions: For IHC/IF: Use at 1:1000 - 1:2500, for WB: Use at 1:2500 - 1:5000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS + sodium azide (0.02%, w/v)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

IgY fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofilament triplet M protein, NEFM, NF-M, Neurofilament 3, NFM, Neurofilament medium polypeptide, Neurofilament3, NEF3, 160 kDa neurofilament protein, NF M

Cellular Localization

Cytoplasmic

Background

Human Neurofilament Medium Chain (NF-M) is a 102,448 dalton protein (916 amino acids) expressed in CNS and PNS neurons. This cytoskeletal protein is normally phosphorylated in vivo, and its extent of phosphorylation correlates with different states of axonal growth and stabilization. These observations have led to the hypothesis that the NF-M protein cross-links adjacent neurofilament strands, and contributes to the structural integrity of the axon.

Database

Research Area

DATA IMAGES
NF-M-antibody-GTX85461-IHC-1_18122102_212.jpg

GTX85461 IHC Image

Dissociated cell culture prepared from an adult rat brain. NF-M (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.

GTX85461__20161004_IFA_18122102_136.jpg

GTX85461 ICC/IF Image

NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250.
Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

NF-M-antibody__GTX85461-1_18122101_720.jpg

GTX85461 ICC/IF Image

Dissociated cell culture prepared from an adult rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to co-stain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining

REFERENCE
Package List Price ($)
$ 289