ICC/IF analysis of U251 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

IHC-P analysis of human colon carcinoma tissue using GTX22722 NFAT1 antibody [25A10.D6.D2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

ICC/IF analysis of HeLa cells untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours using GTX22722 NFAT1 antibody [25A10.D6.D2].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

IHC-P analysis of human tonsil tissue using GTX22722 NFAT1 antibody [25A10.D6.D2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX22722 NFAT1 antibody [25A10.D6.D2]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

ICC/IF analysis of MCF-7 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

IHC-P analysis of human spleen tissue using GTX22722 NFAT1 antibody [25A10.D6.D2].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100