APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 3-6μg/ml |
|
Note :
IHC-P
Human Spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88316-PEP).
Product Note
This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QQFRTGLSSPDARYQ, from the internal region of the protein sequence according to NP_036472.2; NP_775114.1; NP_001129493.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear factor of activated T cells 2 , NFAT1 , NFATP
Cellular Localization
Cytoplasm , Nucleus
Background
This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
|
GTX88316 WB Image
WB analysis of Molt-4 cell lysate using GTX88316 NFAT1 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88316 IHC-P Image
IHC-P analysis of human spleen using GTX88316 NFAT1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for NFAT1 antibody, Internal (GTX88316). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NFAT1 antibody, Internal (GTX88316). Be the first to share your experience with this product.
