GeneTex
United States (US)

NFAT1 antibody, Internal

GTX88316_986_IHC-P_18122102_266.jpg
GTX88316_2381_WB_18122102_127.jpg

Cat No. GTX88316

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3-6μg/ml

Note :

IHC-P
Human Spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes.

Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88316-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QQFRTGLSSPDARYQ, from the internal region of the protein sequence according to NP_036472.2; NP_775114.1; NP_001129493.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Factor Of Activated T Cells 2,Nfat1,Nfatp,Nfatc2

Cellular Localization

Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calciu

Background

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES
GTX88316_986_IHC-P_18122102_266.jpg

GTX88316 IHC-P Image

IHC-P analysis of human spleen using GTX88316 NFAT1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88316_2381_WB_18122102_127.jpg

GTX88316 WB Image

WB analysis of Molt-4 cell lysate using GTX88316 NFAT1 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329