GeneTex
United States (US)

NFAT1 antibody

Comparison
GTX127932_41164_20180420_WB_competitor_watermark_18121410_696.jpg
GTX127932_41164_WB_18121410_718.jpg
GTX127932_41164_IFA_18121410_498.jpg
GTX127932_41164_IP_18121410_539.jpg
GTX127932_41164_WB_M_18121410_426.jpg

Cat No. GTX127932

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

HepG2 , Juarkat , Raji , BCL-1 , Raw264.7

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NFAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NFAT1 antibody, NFATP antibody, NFATC2 antibody, nuclear factor of activated T-cells, preexisting component antibody, NFAT transcription complex, preexisting component antibody, T-cell transcription factor NFAT1 antibody, preexisting nuclear factor of activated T-cells 2 antibody, NF-ATc2 antibody, NFAT pre-existing subunit antibody, nuclear factor of activated T-cells, cytoplasmic 2 antibody, T cell transcription factor NFAT1 antibody, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX127932_41164_20180420_WB_competitor_watermark_18121410_696.jpg

GTX127932 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFAT1 antibody (GTX127932) diluted at 1:500 and competitor's antibody (MA1-025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX127932_41164_WB_18121410_718.jpg

GTX127932 WB Image

NFAT1 antibody detects NFATC2 protein by western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
7.5% SDS-PAGE
NFAT1 antibody (GTX127932) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127932_41164_IFA_18121410_498.jpg

GTX127932 ICC/IF Image

NFAT1 antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis.
Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFAT1 protein stained by NFAT1 antibody (GTX127932) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX127932_41164_IP_18121410_539.jpg

GTX127932 IP Image

NFAT1 antibody immunoprecipitates NFAT1 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of NFAT1 protein by 4 μg NFAT1 antibody (GTX127932)
7.5 % SDS-PAGE
The immunoprecipitated NFAT1 protein was detected by NFAT1 antibody (GTX127932) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX127932_41164_WB_M_18121410_426.jpg

GTX127932 WB Image

NFAT1 antibody detects NFATC2 protein by western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
B. 30 µg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
NFAT1 antibody (GTX127932) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169