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NFAT1 antibody

Comparison
Anti-NFAT1 antibody used in Western Blot (WB). GTX127932
Anti-NFAT1 antibody used in Western Blot (WB). GTX127932
Anti-NFAT1 antibody used in Immunoprecipitation (IP). GTX127932
Anti-NFAT1 antibody used in Western Blot (WB). GTX127932
Anti-NFAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127932

Cat. No. GTX127932

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NFAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

BCL-1 , Raw264.7 , Jurkat , Raji , HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NFAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885655

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 , AI607462 , NF-ATc2 , NF-ATp , NFAT1 , NFAT1-D , Nfatp

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-NFAT1 antibody used in Western Blot (WB). GTX127932

GTX127932 WB Image

NFAT1 antibody detects NFATC2 protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
NFAT1 antibody (GTX127932) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFAT1 antibody used in Western Blot (WB). GTX127932

GTX127932 WB Image

NFAT1 antibody detects NFATC2 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
7.5% SDS-PAGE
NFAT1 antibody (GTX127932) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFAT1 antibody used in Immunoprecipitation (IP). GTX127932

GTX127932 IP Image

NFAT1 antibody immunoprecipitates NFAT1 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of NFAT1 protein by 4 μg NFAT1 antibody (GTX127932)
7.5 % SDS-PAGE
The immunoprecipitated NFAT1 protein was detected by NFAT1 antibody (GTX127932) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-NFAT1 antibody used in Western Blot (WB). GTX127932

GTX127932 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFAT1 antibody (GTX127932) diluted at 1:500 and competitor's antibody (MA1-025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NFAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127932

GTX127932 ICC/IF Image

NFAT1 antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis.
Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFAT1 protein stained by NFAT1 antibody (GTX127932) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Nicola L. Stevenson et al. bioRxiv 2019; (Epub) Regulator of calcineurin-2 is a centriolar protein with a role in cilia length control.
Application : ICC/IF
Reactivity : Human
Stevenson NL et al. J Cell Sci 2018; 131 (9) Regulator of calcineurin-2 is a centriolar protein with a role in cilia length control.
Zhao N et al. Sci Rep 2015; 5 : 17381 Lovastatin blocks Kv1.3 channel in human T cells: a new mechanism to explain its immunomodulatory properties.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169