GeneTex
United States (US)

NFAT1 (phospho Ser54) antibody

Orthogonal
GTX25246_2114_WB_19011519_845.jpg
GTX25246_1762_WB_19011519_667.jpg
GTX25246_634_ICC-IF_19011519_637.jpg

Cat No. GTX25246

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

100 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of mouse NFAT1 that contains serine 54.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AI607462,NF-ATc2,NF-ATp,NFAT1,NFAT1-D,Nfatp

Cellular Localization

Cytoplasm,Nucleus

Background

Nuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT 1 (also known as NFATc2 or NFATp) is a 154 kDa member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT 1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT 1 has been shown to be important in the regulation of its transcriptional activity.

Database

Research Area

DATA IMAGES
GTX25246_2114_WB_19011519_845.jpg

GTX25246 WB Image

Orthogonal

WB analysis of murine T cells treated with PMA and Ca²⁺ ionophore ionomycin (Lane 2-5) using GTX25246 NFAT1 (phospho Ser54) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine containing peptide(Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

GTX25246_1762_WB_19011519_667.jpg

GTX25246 WB Image

Orthogonal

WB analysis of whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1) treated for 20 minutes with 200 nM of PMA (Lane 2), Jurkat (Lane 3) treated for 20 minutes with 200 nM of PMA (Lane 4) using GTX25246 NFAT1 (phospho Ser54) antibody.
Dilution : 1:500-1:2000

GTX25246_634_ICC-IF_19011519_637.jpg

GTX25246 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells treated with 200nM of PMA for 20 minutes using GTX25246 NFAT1 (phospho Ser54) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

REFERENCE
Package List Price ($)
$ 319