WB analysis of whole cell extracts (30 μg lysate) of NIH/3T3 (Lane 1) treated for 20 minutes with 200 nM of PMA (Lane 2), Jurkat (Lane 3) treated for 20 minutes with 200 nM of PMA (Lane 4) using GTX25246 NFAT1 (phospho Ser54) antibody.
Dilution : 1:500-1:2000

ICC/IF analysis of HeLa cells treated with 200nM of PMA for 20 minutes using GTX25246 NFAT1 (phospho Ser54) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

WB analysis of murine T cells treated with PMA and Ca²⁺ ionophore ionomycin (Lane 2-5) using GTX25246 NFAT1 (phospho Ser54) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine containing peptide(Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.