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NFAT2 antibody [AT1C3]

Anti-NFAT2 antibody [AT1C3] used in Western Blot (WB). GTX50030

Cat. No. GTX50030

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1C3

Isotype

 IgG2a

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all NFAT2 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

101 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NFAT2 (428-716aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_11168934

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells 1 , NF-ATC , NF-ATc1.2 , NFAT2 , NFATc

Cellular Localization

Cytoplasm , Nucleus

Background

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-NFAT2 antibody [AT1C3] used in Western Blot (WB). GTX50030

GTX50030 WB Image

Cell lysates of Ramos (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NFATc1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

Application Reference

Seite JF et al. J Allergy Clin Immunol 2014; 133 (1) : 181-8.e1-9 Intravenous immunoglobulin induces a functional silencing program similar to anergy in human B cells.
Application : WB
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399