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NFAT2 antibody

Anti-NFAT2 antibody used in Western Blot (WB). GTX45916
Anti-NFAT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45916
Anti-NFAT2 antibody used in Western Blot (WB). GTX45916
Anti-NFAT2 antibody used in Western Blot (WB). GTX45916

Cat No. GTX45916

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 3 μg/ml
ICC/IF 1:20
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Product Note

The amino acid sequence used is 90% homologous in mouse and rat and 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear factor of activated T cells 1 , NF-ATC , NF-ATc1.2 , NFAT2 , NFATc

Cellular Localization

Cytoplasm,Nucleus

Background

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NFAT2 antibody used in Western Blot (WB). GTX45916

GTX45916 WB Image

WB analysis of Ramos cell lysate using GTX45916 NFAT2 antibody.
Lane 1 : Without blocking peptide
Lane 2 : With blocking peptide

Anti-NFAT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45916

GTX45916 ICC/IF Image

ICC/IF analysis of RAW264.7 cells using GTX45916 NFAT2 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

Anti-NFAT2 antibody used in Western Blot (WB). GTX45916

GTX45916 WB Image

WB analysis of RAW264.7 cell lysate using GTX45916 NFAT2 antibody.

Anti-NFAT2 antibody used in Western Blot (WB). GTX45916

GTX45916 WB Image

WB analysis of Ramos (A) and RAW264.7 (B) cell lysate using GTX45916 NFAT2 antibody.
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379