Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nuclear factor of activated T cells 5 , NF-AT5 , NFATL1 , NFATZ , OREBP , TONEBP
Cellular Localization
Nucleus,Cytoplasm
Background
The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
IHC-P analysis of human skeletal muscle tissue using GTX23446 NFAT5 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX23446 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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GTX23446 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4ºC
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GTX23446 IHC-P Image
IHC-P analysis of human brain tissue using GTX23446 NFAT5 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX23446 WB Image
WB analysis of 25 ug of various whole cell lysates using GTX23446 NFAT5 antibody. Dilution : 1:1000
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GTX23446 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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GTX23446 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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GTX23446 IP Image
IP analysis of U2OS cell lysates using GTX23446 NFAT5 antibody. IP reaction : 3μg antibody / 500μg lysate
