APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2.5-3.8μg/ml |
|
Note :
IHC-P
Human Placenta shows weak nuclear staining in trophoblasts..
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89854-PEP).
Product Note
This antibody is expected to recognise isoform 2, 3, 4 and 5 (NP_004545.2; NP_001185894.1; NP_001185895.1; NP_001185896.1 respectively).
Predict Reactivity
Mouse, Rat, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SGFPAPPGEEPPA, from the C Terminus of the protein sequence according to NP_004545.2; NP_001185894.1; NP_001185895.1; NP_001185896.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear factor of activated T cells 4 , NF-AT3 , NF-ATC4 , NFAT3
Cellular Localization
Cytoplasm, cytosol , Nucleus
Background
This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
|
GTX89854 IHC-P Image
IHC-P analysis of human placenta using GTX89854 NFATC4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
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REVIEW
There are currently no reviews for NFATC4 antibody, C-term (GTX89854). Be the first to share your experience with this product.
