ICC/IF analysis of HeLa cells using GTX23447 NFATC4 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of A431 Cells using GTX23447 NFATC4 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of U251 cells using GTX23447 NFATC4 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX23447 NFATC4 antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

IP analysis of MCF7 cell lysates using GTX23447 NFATC4 antibody.
IP reaction : 3μg antibody / 500μg lysate

WB analysis of 25 ug of various whole cell lysates using GTX23447 NFATC4 antibody.
Dilution : 1:1000