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NFATC4 antibody

Anti-NFATC4 antibody used in Western Blot (WB). GTX23447
Anti-NFATC4 antibody used in Immunoprecipitation (IP). GTX23447
Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447
Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447
Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447
Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447

Cat. No. GTX23447

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all NFATC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IP 3 μg
IHC 1:3000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells 4 , NF-AT3 , NF-ATC4 , NFAT3

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-NFATC4 antibody used in Western Blot (WB). GTX23447

GTX23447 WB Image

WB analysis of 25 ug of various whole cell lysates using GTX23447 NFATC4 antibody.
Dilution : 1:1000

Anti-NFATC4 antibody used in Immunoprecipitation (IP). GTX23447

GTX23447 IP Image

IP analysis of MCF7 cell lysates using GTX23447 NFATC4 antibody.
IP reaction : 3μg antibody / 500μg lysate

Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447

GTX23447 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23447 NFATC4 antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447

GTX23447 ICC/IF Image

ICC/IF analysis of U251 cells using GTX23447 NFATC4 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447

GTX23447 ICC/IF Image

ICC/IF analysis of A431 Cells using GTX23447 NFATC4 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-NFATC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23447

GTX23447 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23447 NFATC4 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399