GeneTex
United States (US)

NFIB antibody [N1C2]

GTX105749_39757_IHC_M_2_19011506_564.jpg
GTX105749_39757_WB_M_brain_19011506_579.jpg
GTX105749_39757_IHC_M_19011506_208.jpg
GTX105749_WB_19011506_708.jpg

Cat No. GTX105749

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

nuclear factor I/B tansfected 293T cell , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HMGIC/NFIB antibody, NFI-RED antibody, NFIB2 antibody, NFIB3 antibody, NFIB antibody, nuclear factor 1 B-type antibody, CCAAT-box-binding transcription factor antibody, TGGCA-binding protein antibody, nuclear factor 1/B antibody, nuclear factor I/B antibody

Cellular Localization

Nucleus

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Database

Research Area

DATA IMAGES
GTX105749_39757_IHC_M_2_19011506_564.jpg

GTX105749 IHC-P Image

NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NFIB antibody [N1C2] (GTX105749) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105749_39757_WB_M_brain_19011506_579.jpg

GTX105749 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105749 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105749_39757_IHC_M_19011506_208.jpg

GTX105749 IHC-P Image

NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NFIB antibody [N1C2] (GTX105749) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105749_WB_19011506_708.jpg

GTX105749 WB Image

Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody.
A: Non-transfected lysate.
B: NFIB transfected lysate.
12% SDS PAGE
GTX105749 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169