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NFIX antibody [HL1269]

Recombinant antibody
Anti-NFIX antibody [HL1269] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636671
Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671
Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671
Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671
Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671

Cat. No. GTX636671

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1269

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NFIX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Human, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910039

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor I X , CTF , MRSHSS , NF-I/X , NF1-X , NF1A , SOTOS2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-NFIX antibody [HL1269] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636671

GTX636671 ICC/IF Image

NFIX antibody [HL1269] detects NFIX protein at nucleus by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFIX stained by NFIX antibody [HL1269] (GTX636671) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671

GTX636671 IHC-P Image

NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
NFIX stained by NFIX antibody [HL1269] (GTX636671) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671

GTX636671 IHC-P Image

NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NFIX stained by NFIX antibody [HL1269] (GTX636671) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671

GTX636671 IHC-P Image

NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NFIX stained by NFIX antibody [HL1269] (GTX636671) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFIX antibody [HL1269] used in IHC (Paraffin sections) (IHC-P). GTX636671

GTX636671 IHC-P Image

NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NFIX stained by NFIX antibody [HL1269] (GTX636671) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations