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NFIX antibody [N2C2], Internal

Anti-NFIX antibody [N2C2], Internal used in Western Blot (WB). GTX118630
Anti-NFIX antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX118630

Cat No. GTX118630

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Factor I X,Ctf,Mrshss,Nf-I/X,Nf1-X,Nf1A,Sotos2,Nfix

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Database

DATA IMAGES
Anti-NFIX antibody [N2C2], Internal used in Western Blot (WB). GTX118630

GTX118630 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
10% SDS PAGE
GTX118630 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFIX antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX118630

GTX118630 IHC-P Image

NFIX antibody [N2C2], Internal detects NFIX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
NFIX stained by NFIX antibody [N2C2], Internal (GTX118630) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169