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NFS1 antibody

Anti-NFS1 antibody used in Western Blot (WB). GTX33356
Anti-NFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33356
Anti-NFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33356
Anti-NFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33356
Anti-NFS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33356

Cat. No. GTX33356

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NFS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFS1 cysteine desulfurase , HUSSY-08 , IscS , NIFS

Cellular Localization

Nucleus

Background

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-NFS1 antibody used in Western Blot (WB). GTX33356

GTX33356 WB Image

WB analysis of various sample lysates using GTX33356 NFS1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33356

GTX33356 ICC/IF Image

ICC/IF analysis of U-251 MG cells using GTX33356 NFS1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33356

GTX33356 ICC/IF Image

ICC/IF analysis of L929 cells using GTX33356 NFS1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33356

GTX33356 ICC/IF Image

ICC/IF analysis of C6 cells using GTX33356 NFS1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NFS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33356

GTX33356 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33356 NFS1 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Florian A Schober et al. Sci Adv 2021; 7 (8) The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399