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NFYA antibody, C-term

Anti-NFYA antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77848
Anti-NFYA antibody, C-term used in Western Blot (WB). GTX77848

Cat. No. GTX77848

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all NFYA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human NFYA

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_424796

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear transcription factor Y subunit alpha , CBF-A , CBF-B , HAP2 , NF-YA

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NFYA antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77848

GTX77848 IHC-P Image

IHC-P analysis of human liver tissue using GTX77848 NFYA antibody at 4.0-8.0μg/ml.

Anti-NFYA antibody, C-term used in Western Blot (WB). GTX77848

GTX77848 WB Image

WB analysis of Jurkat cells using GTX77848 NFYA antibody at 5.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399