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NFYA antibody

Anti-NFYA antibody used in IHC (Paraffin sections) (IHC-P). GTX48667
Anti-NFYA antibody used in Western Blot (WB). GTX48667
Anti-NFYA antibody used in IHC (Paraffin sections) (IHC-P). GTX48667

Cat. No. GTX48667

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, EMSA

Reactivity

 Human
Package
50 μl ($399)
See all NFYA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
IHC-P 1:400
ELISA 1:20000-1:100000
EMSA 2 μL
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filtered-steriled serum

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear transcription factor Y subunit alpha , CBF-A , CBF-B , HAP2 , NF-YA

Cellular Localization

Nucleus

Background

Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.

Database

Research Area

DATA IMAGES

Anti-NFYA antibody used in IHC (Paraffin sections) (IHC-P). GTX48667

GTX48667 IHC-P Image

GeneTex's anti-NF-Y (A Subunit) antibody was diluted at 10 μg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.

Anti-NFYA antibody used in Western Blot (WB). GTX48667

GTX48667 WB Image

CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Load: 35 μg per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.

Anti-NFYA antibody used in IHC (Paraffin sections) (IHC-P). GTX48667

GTX48667 IHC-P Image

IHC-P analysis of human prostate tissue using GTX48667 NFYA antibody.
Dilution : 10 μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399