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NFkB p100 antibody [C2C3], C-term

Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150
Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150
Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150
Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150
Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150

Cat No. GTX101150

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , NIH-3T3 ,*YY-8103

Specificity/Sensitivity

GTX101150 specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform.

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Factor Kappa B Subunit 2,Cvid10,H2Tf1,Lyt-10,Lyt10,Nf-Kb2,P100,P49/P100,P52,Nfkb2

Cellular Localization

Nucleus , Cytoplasm

Background

NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150

GTX101150 WB Image

NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.

Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150

GTX101150 WB Image

Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The CRISPR knockout cell line was generated by EdiGene Inc.

Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150

GTX101150 IP Image

NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150

GTX101150 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX101150 diluted at 1:1000

Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150

GTX101150 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
NFkB p100 antibody
GTX101150 diluted at 1:1000

REFERENCE
There are currently no references for NFkB p100 antibody [C2C3], C-term (GTX101150). Be the first to share your publications with this product.
REVIEW

NFkB p100 antibody [C2C3], C-term Cat No. GTX101150

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 09-Apr-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : YY-8103 cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:500, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169