APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2 , NIH-3T3 , A431
Product Note
GTX101150 specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform.
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241105
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear factor kappa B subunit 2 , CVID10 , H2TF1 , LYT-10 , LYT10 , NF-kB2 , p100 , p49/p100 , p52
Cellular Localization
Nucleus , Cytoplasm
Background
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX101150 WB Image
NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.
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GTX101150 IP Image
NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101150 WB Image
Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101150 WB Image
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE GTX101150 diluted at 1:1000
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GTX101150 WB Image
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) 7.5% SDS PAGE NFkB p100 antibody GTX101150 diluted at 1:1000
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REFERENCE
There are currently no references for NFkB p100 antibody [C2C3], C-term (GTX101150). Be the first to share your publications with this product.
REVIEW
NFkB p100 antibody [C2C3], C-term
Cat. No. GTX101150
Rating
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( Average 4 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 09-Apr-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
YY-8103 cells
Amount used :
30μg
Blocking :
5% non-fat milk, 25°C, 0.5Hr
Primary Antibody :
1:500, 25°C, 2Hr