NFkB p100 antibody [C2C3], C-term

Name: NFkB p100 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX101150
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150

Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150

Cat. No. GTX101150

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all NFkB p100 products

Datasheet
Document

View product citations for antibody GTX101150 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa , HepG2 , NIH-3T3 , A431

Product Note

GTX101150 specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform.

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241105

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 2 , CVID10 , H2TF1 , LYT-10 , LYT10 , NF-kB2 , p100 , p49/p100 , p52

Cellular Localization

Nucleus , Cytoplasm

Background

NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM]

Database

Gene ID: 4791 NFKB2
UniProt: Q00653 NFKB2

Research Area

DATA IMAGES

GTX101150 WB Image

NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.

GTX101150 IP Image

NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101150 WB Image

Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101150 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX101150 diluted at 1:1000

GTX101150 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
NFkB p100 antibody
GTX101150 diluted at 1:1000

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REVIEW