GeneTex
United States (US)

NFkB p100 antibody [C2C3], C-term

KO/KD
GTX101150_39549_20150924_WB_19011506_917.jpg
GTX101150_39549_20170427_WB_KO_watermark_19011506_611.jpg
GTX101150_39549_IP_19011506_589.jpg
GTX101150_WB_M_19011506_461.jpg
GTX101150_WB_19011506_524.jpg

Cat No. GTX101150

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , NIH-3T3 ,*YY-8103

Specificity/Sensitivity

GTX101150 specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform.

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Factor Kappa B Subunit 2,Cvid10,H2Tf1,Lyt-10,Lyt10,Nf-Kb2,P100,P49/P100,P52,Nfkb2

Cellular Localization

Nucleus , Cytoplasm

Background

NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101150_39549_20150924_WB_19011506_917.jpg

GTX101150 WB Image

NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.

GTX101150_39549_20170427_WB_KO_watermark_19011506_611.jpg

GTX101150 WB Image

KO/KD

Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101150_39549_IP_19011506_589.jpg

GTX101150 IP Image

NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101150_WB_M_19011506_461.jpg

GTX101150 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX101150 diluted at 1:1000

GTX101150_WB_19011506_524.jpg

GTX101150 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
NFkB p100 antibody
GTX101150 diluted at 1:1000

REVIEW

NFkB p100 antibody [C2C3], C-term Cat No. GTX101150

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 09-Apr-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : YY-8103 cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:500, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169