APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Mouse, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NFkB p100/p52. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10614977
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear factor kappa B subunit 2 , CVID10 , H2TF1 , LYT-10 , LYT10 , NF-kB2 , p100 , p49/p100 , p52
Cellular Localization
Nucleus , Cytoplasm
Background
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-NFkB p100/p52 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101151](/templates/default/js/lightbox/images/loading.gif "Anti-NFkB p100/p52 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101151")
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GTX101151 IHC-P Image
NFkB p100/p52 antibody [N1N3] detects NFKB2 protein at cytoplasm in rat thymus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
NFkB p100/p52 antibody [N1N3] (GTX101151) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for NFkB p100/p52 antibody [N1N3] (GTX101151). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NFkB p100/p52 antibody [N1N3] (GTX101151). Be the first to share your experience with this product.
![Anti-NFkB p100/p52 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101151](/upload/website/prouct_img/small/GTX101151/GTX101151_40079_20151123_IHC-P_R_w_23060100_646.webp "Anti-NFkB p100/p52 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101151")
![Anti-NFkB p100/p52 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101151](/upload/website/prouct_img/normal/GTX101151/GTX101151_40079_20151123_IHC-P_R_w_23060100_646.webp "Anti-NFkB p100/p52 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101151")