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NFkB p100 (phospho Ser865) antibody

Anti-NFkB p100 (phospho Ser865) antibody used in IHC (Paraffin sections) (IHC-P). GTX79000
Anti-NFkB p100 (phospho Ser865) antibody used in Western Blot (WB). GTX79000

Cat No. GTX79000

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p100/p52 around the phosphorylation site of serine 865 (E-D-Sp-A-Y).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CVID10,H2TF1,LYT10,NFKB2,NFkB2,nuclear factor kappa B subunit 2,p100,p49/p100,p52,NFkB p100 / p52,NFkB p100,NF-κb p100,NF-κb p52

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-NFkB p100 (phospho Ser865) antibody used in IHC (Paraffin sections) (IHC-P). GTX79000

GTX79000 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79000 NFkB p100 (phospho Ser865) antibody.

Anti-NFkB p100 (phospho Ser865) antibody used in Western Blot (WB). GTX79000

GTX79000 WB Image

WB analysis of ovary cancer cell lysate using GTX79000 NFkB p100 (phospho Ser865) antibody.

REFERENCE

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REVIEW

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