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NFkB p105 antibody

KO/KD
Anti-NFkB p105 antibody used in ChIP assay (ChIP assay). GTX110585
Anti-NFkB p105 antibody used in Western Blot (WB). GTX110585
Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585
Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585
Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585
Anti-NFkB p105 antibody used in Western Blot (WB). GTX110585
Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585
Anti-NFkB p105 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110585
Anti-NFkB p105 antibody used in Western Blot (WB). GTX110585

Cat. No. GTX110585

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ChIP assay, IHC

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all NFkB p105 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFkB p105. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950995

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NFkB p105 antibody used in ChIP assay (ChIP assay). GTX110585

GTX110585 ChIP assay Image

NFkB p105 antibody immunoprecipitates NFkB p105 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of NFkB p105 antibody (GTX110585) The precipitated DNA was detected by PCR with primer set targeting to PPM1D promoter.

Anti-NFkB p105 antibody used in Western Blot (WB). GTX110585

GTX110585 WB Image

KO/KD

Wild-type (WT) and NFkB p105 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p105 antibody (GTX110585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585

GTX110585 IHC-P Image

NFkB p105 antibody detects secreted NFkB p105 protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog colon.
NFkB p105 stained by NFkB p105 antibody (GTX110585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585

GTX110585 IHC-P Image

NFkB p105 antibody detects NFkB p105 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
NFkB p105 stained by NFkB p105 antibody (GTX110585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585

GTX110585 IHC-P Image

NFkB p105 antibody detects NFkB p105 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
NFkB p105 stained by NFkB p105 antibody (GTX110585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p105 antibody used in Western Blot (WB). GTX110585

GTX110585 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p105 antibody (GTX110585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX110585

GTX110585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NFkB p105 (GTX110585) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NFkB p105 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110585

GTX110585 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFkB p105(GTX110585) antibody at 1:200 dilution.

Anti-NFkB p105 antibody used in Western Blot (WB). GTX110585

GTX110585 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX110585 diluted at 1:1000

REFERENCE

Application Reference

Yang HL et al. Aging (Albany NY) 2021; 13 (12) : 15964-15989 Antrodia salmonea induces apoptosis and enhances cytoprotective autophagy in colon cancer cells.
Reactivity : Human
Koya T et al. Am J Physiol Heart Circ Physiol 2012; 303 (1) : H96-105 Salusin-β accelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro.
Application : IHC
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169