NFkB p105/p50 antibody [5D10]

Cat. No. GTX60455

Host	Mouse
Clonality	Monoclonal
Clone Name	5D10
Isotype	IgG2a
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

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Datasheet
Document

View product citations for antibody GTX60455 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NFkB1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Gene ID: 4790 NFKB1
UniProt: P19838 NFKB1

Research Area

DATA IMAGES

GTX60455 WB Image

WB analysis of K562 (1), Jurkat (2), A431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate using GTX60455 NFkB p105/p50 antibody [5D10].

GTX60455 FACS Image

FACS analysis of MCF-7 cells using GTX60455 NFkB p105/p50 antibody [5D10].
Green : NFkB p105/p50
Purple : negative control

GTX60455 ELISA Image

ELISA analysis of antigen using GTX60455 NFkB p105/p50 antibody [5D10].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60455 IHC-P Image

IHC-P analysis of prostate tissue (left) and bladder cancer tissue (right) using GTX60455 NFkB p105/p50 antibody [5D10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty