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NFkB p105/p50 antibody [5D10D11]

Anti-NFkB p105/p50 antibody [5D10D11] used in ELISA (ELISA). GTX60465
Anti-NFkB p105/p50 antibody [5D10D11] used in IHC (Paraffin sections) (IHC-P). GTX60465
Anti-NFkB p105/p50 antibody [5D10D11] used in Western Blot (WB). GTX60465
Anti-NFkB p105/p50 antibody [5D10D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60465
Anti-NFkB p105/p50 antibody [5D10D11] used in Flow cytometry (FACS). GTX60465

Cat. No. GTX60465

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D10D11

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($409)
See all NFkB p105/p50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NFkB1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-NFkB p105/p50 antibody [5D10D11] used in ELISA (ELISA). GTX60465

GTX60465 ELISA Image

ELISA analysis of antigen using GTX60465 NFkB p105/p50 antibody [5D10D11].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-NFkB p105/p50 antibody [5D10D11] used in IHC (Paraffin sections) (IHC-P). GTX60465

GTX60465 IHC-P Image

IHC-P analysis of human intima cancer tissue (left) and human bladder cancer tissue (right) using GTX60465 NFkB p105/p50 antibody [5D10D11].

Anti-NFkB p105/p50 antibody [5D10D11] used in Western Blot (WB). GTX60465

GTX60465 WB Image

WB analysis of K562 (1), Jurkat (2), A431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate using GTX60465 NFkB p105/p50 antibody [5D10D11].

Anti-NFkB p105/p50 antibody [5D10D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60465

GTX60465 ICC/IF Image

ICC/IF analysis of U251 cells using GTX60465 NFkB p105/p50 antibody [5D10D11].
Green : NFkB p105/p50
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-NFkB p105/p50 antibody [5D10D11] used in Flow cytometry (FACS). GTX60465

GTX60465 FACS Image

FACS analysis of MCF-7 cells using GTX60465 NFkB p105/p50 antibody [5D10D11].
Green : NFkB p105/p50
Purple : negative control

REFERENCE

Application Reference

Andy Darma et al. Iran J Microbiol 2020; 12 (5) : 445-450 Effects of probiotics on the enhancement of the innate mucosal immune response against pathogenic bacteria.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409