WB analysis of HeLa cells treated by TNF-α (20 ng/mL) and Calyculin A (100 nM) at 37ºC for 10 minutes and C6 cells treated by TNF-α (20 ng/mL) at 37ºC for 10 minutes using GTX54547 NFkB p105/p50 (phospho Ser337) antibody.
Dilution : 1:1000
Loading : 25μg per lane