APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 337 (R-K-Sp-D-L).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11172261
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CVID12,EBP1,KBF1,NFKB1,NFKBp105,NFKBp50,NFkB1,NFkappaB,nuclear factor kappa B subunit 1,p105,p50,NFkB p105 / p50,NFkB p105,NF-κb p105,NF-κb p50
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX79002 WB Image
WB analysis of HeLa cells treated with TNF-α lysate using GTX79002 NFkB p105/p50 (phospho Ser337) antibody.
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GTX79002 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX79002 NFkB p105/p50 (phospho Ser337) antibody.
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REFERENCE
There are currently no references for NFkB p105/p50 (phospho Ser337) antibody (GTX79002). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NFkB p105/p50 (phospho Ser337) antibody (GTX79002). Be the first to share your experience with this product.
