APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 - 1:1000 |
| 1:100 - 1:500 |
| 1:100 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of NFkB p105. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX32222 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32222 NFkB p105 (phospho Ser927) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
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GTX32222 WB Image
WB analysis of UV-treated HeLa (A), A2780 (B), NIH3T3 (C), TNFa-treated Raw264.7 (D), rat liver (E), rat kidney (F) whole cell lysates using GTX32222 NFkB p105 (phospho Ser927) antibody.
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GTX32222 ICC/IF Image
ICC/IF analysis of formalin-fixed HeLa cells using GTX32222 NFkB p105 (phospho Ser927) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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REFERENCE
There are currently no references for NFkB p105 (phospho Ser927) antibody (GTX32222). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NFkB p105 (phospho Ser927) antibody (GTX32222). Be the first to share your experience with this product.
