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NFkB p105 (phospho Ser927) antibody

Anti-NFkB p105 (phospho Ser927) antibody used in IHC (Paraffin sections) (IHC-P). GTX32222
Anti-NFkB p105 (phospho Ser927) antibody used in Western Blot (WB). GTX32222
Anti-NFkB p105 (phospho Ser927) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32222

Cat No. GTX32222

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

105 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of NFkB p105. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES
Anti-NFkB p105 (phospho Ser927) antibody used in IHC (Paraffin sections) (IHC-P). GTX32222

GTX32222 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32222 NFkB p105 (phospho Ser927) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-NFkB p105 (phospho Ser927) antibody used in Western Blot (WB). GTX32222

GTX32222 WB Image

WB analysis of UV-treated HeLa (A), A2780 (B), NIH3T3 (C), TNFa-treated Raw264.7 (D), rat liver (E), rat kidney (F) whole cell lysates using GTX32222 NFkB p105 (phospho Ser927) antibody.

Anti-NFkB p105 (phospho Ser927) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32222

GTX32222 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32222 NFkB p105 (phospho Ser927) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE
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REVIEW
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