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NFkB p105 (phospho Ser927) antibody

Anti-NFkB p105 (phospho Ser927) antibody used in Western Blot (WB). GTX50324
Anti-NFkB p105 (phospho Ser927) antibody used in IHC (Paraffin sections) (IHC-P). GTX50324
Anti-NFkB p105 (phospho Ser927) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50324

Cat No. GTX50324

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 927 (C-D-S(p)-G-V) derived from human NFkB p105.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-NFkB p105 (phospho Ser927) antibody used in Western Blot (WB). GTX50324

GTX50324 WB Image

WB analysis of extracts from HL-60 cells untreated or treated with TNF-a using GTX50324 NFkB p105 (phospho Ser927) antibody.

Anti-NFkB p105 (phospho Ser927) antibody used in IHC (Paraffin sections) (IHC-P). GTX50324

GTX50324 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50324 NFkB p105 (phospho Ser927) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-NFkB p105 (phospho Ser927) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50324

GTX50324 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50324 NFkB p105 (phospho Ser927) antibody.

REFERENCE

There are currently no references for NFkB p105 (phospho Ser927) antibody (GTX50324). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NFkB p105 (phospho Ser927) antibody (GTX50324). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319