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NFkB p65 (acetyl Lys310) antibody

Anti-NFkB p65 (acetyl Lys310) antibody used in IHC (Paraffin sections) (IHC-P). GTX86963
Anti-NFkB p65 (acetyl Lys310) antibody used in Western Blot (WB). GTX86963
Anti-NFkB p65 (acetyl Lys310) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX86963

Cat. No. GTX86963

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Monkey
Package
100 μg ($399)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
IHC Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human NFkB p65 (275-324).

Purification

Purified by antigen-affinity chromatography. Non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
From serum

Conjugation

Unconjugated

RRID

AB_10726079

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NFKB3 , RELA , RELA protooncogene, NFkB subunit , p65 , NFkB p65 , NF-κb p65 , v-rel avian reticuloendotheliosis viral oncogene homolog A

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 (acetyl Lys310) antibody used in IHC (Paraffin sections) (IHC-P). GTX86963

GTX86963 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86963 NFkB p65 (acetyl Lys310) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NFkB p65 (acetyl Lys310) antibody used in Western Blot (WB). GTX86963

GTX86963 WB Image

WB analysis of HeLa cells treated with TSA 40nM (24h) lysate using GTX86963 NFkB p65 (acetyl Lys310) antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NFkB p65 (acetyl Lys310) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX86963

GTX86963 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX86963 NFkB p65 (acetyl Lys310) antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Li J et al. Front Immunol 2021; 12 : 649285 Quercetin Attenuates Trauma-Induced Heterotopic Ossification by Tuning Immune Cell Infiltration and Related Inflammatory Insult.
Application : WB
Reactivity : Mouse
Luo X et al. Molecules 2018; 23 (3) Aloin Suppresses Lipopolysaccharide-Induced Inflammatory Response and Apoptosis by Inhibiting the Activation of NF-κB.
Application : ICC/IF
Reactivity : Mouse
Lu J et al. EMBO Rep 2017; (Epub) The phosphorylation status of T522 modulates tissue-specific functions of SIRT1 in energy metabolism in mice.
Application : WB
Reactivity : Mouse
Mohan M et al. J Immunol 2014; 194 (1) : 291-306 Dysregulated miR-34a-SIRT1-Acetyl p65 Axis Is a Potential Mediator of Immune Activation in the Colon during Chronic Simian Immunodeficiency Virus Infection of Rhesus Macaques.
Application : ICC/IF
Reactivity : Monkey
Su BH et al. Nat Commun 2013; 4 : 1906 Prothymosin α overexpression contributes to the development of pulmonary emphysema.
Application : IHC
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399