GeneTex
United States (US)

NFkB p65 (acetyl Lys310) antibody

NFkB-p65-acetyl-Lys310-antibody-GTX86963-WB-1_18121411_648.jpg
NFkB-p65-acetyl-Lys310-antibody-GTX86963-IHC-1_18121411_617.jpg
NFkB-p65-acetyl-Lys310-antibody-GTX86963-ICC_IF-1_18121411_422.jpg

Cat No. GTX86963

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human, Mouse, Monkey
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

NF-kappaB p65 (Acetyl-Lys310) Antibody detects endogenous levels of total NF-kappaB p65 protein only when acetylated at lysine310.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 275 and 324 of human NF-kappaB p65.

Purification

The antibody was purified from rabbit antiserum by affinitychromatography using acetylated peptide. The antibody against nonacetylated peptide was removed by chromatography using corresponding non-acetylated peptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rela Proto-Oncogene, Nf-Kb Subunit,Nfkb3,P65,Rela

Cellular Localization

Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Database

Research Area

DATA IMAGES
NFkB-p65-acetyl-Lys310-antibody-GTX86963-WB-1_18121411_648.jpg

GTX86963 WB Image

Western blot analysis of extracts from HeLa cells, treated with TSA 40nM 24h, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The lane on the right is treated with the synthesized peptide.

NFkB-p65-acetyl-Lys310-antibody-GTX86963-IHC-1_18121411_617.jpg

GTX86963 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is treated with the synthesized peptide.

NFkB-p65-acetyl-Lys310-antibody-GTX86963-ICC_IF-1_18121411_422.jpg

GTX86963 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is treated with the synthesized peptide.

REFERENCE
Package List Price ($)
$ 329