United States (US)

NFkB p65 (acetyl Lys310) antibody


Cat No. GTX86963

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human, Mouse, Monkey

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

60 kDa. ( Note )


NF-kappaB p65 (Acetyl-Lys310) Antibody detects endogenous levels of total NF-kappaB p65 protein only when acetylated at lysine310.




Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species



The immunogen corresponding to a region within amino acids 275 and 324 of human NF-kappaB p65.


The antibody was purified from rabbit antiserum by affinitychromatography using acetylated peptide. The antibody against nonacetylated peptide was removed by chromatography using corresponding non-acetylated peptide.




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Rela Proto-Oncogene, Nf-Kb Subunit,Nfkb3,P65,Rela

Cellular Localization

Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.


NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.


Research Area


GTX86963 WB Image

Western blot analysis of extracts from HeLa cells, treated with TSA 40nM 24h, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The lane on the right is treated with the synthesized peptide.


GTX86963 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is treated with the synthesized peptide.


GTX86963 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 329