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NFkB p65 antibody [112A1021]

Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594
Anti-NFkB p65 antibody [112A1021] used in IHC (Paraffin sections) (IHC-P). GTX13594
Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594
Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594
Anti-NFkB p65 antibody [112A1021] used in IHC (Paraffin sections) (IHC-P). GTX13594
Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594
Anti-NFkB p65 antibody [112A1021] used in Flow cytometry (FACS). GTX13594

Cat. No. GTX13594

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 112A1021

Isotype

 IgG1

Application

 WB, IHC-P, FACS, MS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 5 μg/ml
IHC-P 5 μg/ml
FACS 0.25 - 0.5 μg / 10⁶ cells
MS Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Pig, Monkey, Gorilla(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594

GTX13594 WB Image

WB analysis of Daudi, HeLa cell lysates using GTX13594 NFkB p65 antibody [112A1021].
Dilution : 1 μg/ml

Anti-NFkB p65 antibody [112A1021] used in IHC (Paraffin sections) (IHC-P). GTX13594

GTX13594 IHC-P Image

IHC-P analysis of human ovarian cystadenocarcinoma tissue using GTX13594 NFkB p65 antibody [112A1021].
Dilution : 5 μg/ml

Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594

GTX13594 WB Image

WB analysis of (A) Ramos, B) Daudi, C) HeLa and D) NIH 3T3 cell lysates using GTX13594 NFkB p65 antibody [112A1021].
Loading : 30μg
Dilution : 2 μg/ml

Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594

GTX13594 WB Image

WB analysis of (A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysates using GTX13594 NFkB p65 antibody [112A1021].
Loading : 30μg
Dilution : 2 μg/ml

Anti-NFkB p65 antibody [112A1021] used in IHC (Paraffin sections) (IHC-P). GTX13594

GTX13594 IHC-P Image

IHC-P analysis of human ovarian cystadenocarcinoma tissue using GTX13594 NFkB p65 antibody [112A1021].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

Anti-NFkB p65 antibody [112A1021] used in Western Blot (WB). GTX13594

GTX13594 WB Image

WB analysis of HeLa cell lysate using GTX13594 NFkB p65 antibody [112A1021].

Anti-NFkB p65 antibody [112A1021] used in Flow cytometry (FACS). GTX13594

GTX13594 FACS Image

FACS (Intracellular staining) analysis of HEK293 cells using GTX13594 NFkB p65 antibody [112A1021].
Red : primary antibody
Green : isotype control
Antibody amount : 0.5μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449