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NFkB p65 antibody [GT1185]

Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947
Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947
Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947
Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947
Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947
Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947
Anti-NFkB p65 antibody [GT1185] used in Immunoprecipitation (IP). GTX00947

Cat No. GTX00947

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1185

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human NF-κB p65.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , NFKB3 , RELA , RELA protooncogene, NFkB subunit , p65 , NFkB p65 , NF-κb p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947

GTX00947 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody [GT1185] (GTX00947) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947

GTX00947 WB Image

WB analysis of wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Laoding : 25μg

Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947

GTX00947 WB Image

WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg

Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947

GTX00947 WB Image

WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg

Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947

GTX00947 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100

Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947

GTX00947 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100

Anti-NFkB p65 antibody [GT1185] used in Immunoprecipitation (IP). GTX00947

GTX00947 IP Image

IP analysis of HeLa cell lysate using GTX00947 NFkB p65 antibody [GT1185].
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

There are currently no references for NFkB p65 antibody [GT1185] (GTX00947). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NFkB p65 antibody [GT1185] (GTX00947). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379