APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS (pH7.3), 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human NF-κB p65.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
RELA proto-oncogene, NF-kB subunit , NFKB3 , RELA , RELA protooncogene, NFkB subunit , p65 , NFkB p65 , NF-κb p65
Cellular Localization
Nucleus,Cytoplasm
Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/templates/default/js/lightbox/images/loading.gif "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
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GTX00947 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody [GT1185] (GTX00947) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00947 IHC-P Image
IHC-P analysis of human lung cancer tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100
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GTX00947 IHC-P Image
IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100
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GTX00947 IP Image
IP analysis of HeLa cell lysate using GTX00947 NFkB p65 antibody [GT1185].
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
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GTX00947 WB Image
WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg
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GTX00947 WB Image
WB analysis of wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Laoding : 25μg
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GTX00947 WB Image
WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg
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REFERENCE
There are currently no references for NFkB p65 antibody [GT1185] (GTX00947). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NFkB p65 antibody [GT1185] (GTX00947). Be the first to share your experience with this product.
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_40000000132_20200306_WB_w_23053121_680.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_20200327_IHC-P_14_w_23053121_520.webp "Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_20200327_IHC-P_13_w_23053121_725.webp "Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Immunoprecipitation (IP). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_20210323_IP_2_w_23053121_184.webp "Anti-NFkB p65 antibody [GT1185] used in Immunoprecipitation (IP). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_20200327_WB_2_w_23053121_405.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_20200327_WB_1_w_23053121_277.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/small/GTX00947/GTX00947_20200327_WB_3_w_23053121_334.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_40000000132_20200306_WB_w_23053121_680.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_IHC-P_14_w_23053121_520.webp "Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_IHC-P_13_w_23053121_725.webp "Anti-NFkB p65 antibody [GT1185] used in IHC (Paraffin sections) (IHC-P). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Immunoprecipitation (IP). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_20210323_IP_2_w_23053121_184.webp "Anti-NFkB p65 antibody [GT1185] used in Immunoprecipitation (IP). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_WB_2_w_23053121_405.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_WB_1_w_23053121_277.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")
![Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947](/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_WB_3_w_23053121_334.webp "Anti-NFkB p65 antibody [GT1185] used in Western Blot (WB). GTX00947")