NFkB p65 antibody [GT1185]

Cat. No. GTX00947

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1185
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all NFkB p65 products

Datasheet
Document

View product citations for antibody GTX00947 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200
IP	1:50 - 1:200

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human NF-κB p65.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , NFKB3 , RELA , RELA protooncogene, NFkB subunit , p65 , NFkB p65 , NF-κb p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Gene ID: 5970 RELA
UniProt: Q04206 RELA

Research Area

DATA IMAGES

GTX00947 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody [GT1185] (GTX00947) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX00947 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100

GTX00947 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100

GTX00947 IP Image

IP analysis of HeLa cell lysate using GTX00947 NFkB p65 antibody [GT1185].
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

GTX00947 WB Image

WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg

GTX00947 WB Image

WB analysis of wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Laoding : 25μg

GTX00947 WB Image

WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

NFkB p65 antibody [GT1185]

Cat. No. GTX00947

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX00947 WB Image

GTX00947 IHC-P Image

GTX00947 IHC-P Image

GTX00947 IP Image

GTX00947 WB Image

GTX00947 WB Image

GTX00947 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.