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NFkB p65 antibody

KO/KD Overexpression IP/MS
GTX102090_42270_20170413_WB_KO_watermark_19011506_772.jpg
GTX102090_40275_IHC_19011506_754.jpg
GTX102090_40275_IFA_19011506_263.jpg
GTX102090_42270_20171208_WB_19011506_151.jpg
GTX102090_42270_20151126_WB_B_19011506_454.jpg
GTX102090_40275_20141009_IHC_M_19011506_225.jpg
GTX102090_42438_20160324_WB_19011506_268.jpg

Cat No. GTX102090

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , K562 , THP-1 , HL-60 , human NFkB p65-transfected 293T

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377380)

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rela Proto-Oncogene, Nf-Kb Subunit,Nfkb3,P65,Rela

Cellular Localization

Nucleus , Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102090_42270_20170413_WB_KO_watermark_19011506_772.jpg

GTX102090 WB Image

KO/KD

Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102090_40275_IHC_19011506_754.jpg

GTX102090 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NFkB p65 antibody (GTX102090) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102090_40275_IFA_19011506_263.jpg

GTX102090 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX102090) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX102090_42270_20171208_WB_19011506_151.jpg

GTX102090 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102090_42270_20151126_WB_B_19011506_454.jpg

GTX102090 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102090_40275_20141009_IHC_M_19011506_225.jpg

GTX102090 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NFkB p65 antibody (GTX102090) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102090_42438_20160324_WB_19011506_268.jpg

GTX102090 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000.

REFERENCE
REVIEW

NFkB p65 antibody Cat No. GTX102090

Rating ( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3.5 based on 2 users reviews)
Date : Anonymous submitted on 18-Jul-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : mpkCCD cells
Amount used : 50 μg
Blocking : 0.1%BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 30-Aug-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : Neuro-2a cells
Amount used : 25μg
Blocking : 5% milk in 0.1% PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169