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NFkB p65 antibody

KO/KD Comparison Orthogonal Overexpression IP/MS
Anti-NFkB p65 antibody used in Immunoprecipitation (IP). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107678
Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

Cat. No. GTX107678

Reference 24
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, EMSA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
EMSA Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

GFP-human NF-kappa-B-transfected 293T , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Product Note

IP/MS validation was supported by references (PMID:30377388)

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus , Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 antibody used in Immunoprecipitation (IP). GTX107678

GTX107678 IP Image

NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

KO/KD

Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000 and competitor's antibody (#4764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107678

GTX107678 ICC/IF Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107678

GTX107678 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678

GTX107678 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678

GTX107678 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107678

GTX107678 ICC/IF Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm, nucleus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678

GTX107678 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Overexpression

NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678

GTX107678 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX107678

GTX107678 IHC-P Image

NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2014. PMID: 24723958

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

The data was published in the 2021 in Transl Neurosci. PMID: 33708438

Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678

GTX107678 WB Image

The data was published in the journal Nat Commun in 2018.PMID: 29968710

REFERENCE

Application Reference

HL Yang et al. Int J Biol Sci 2024; 20 (8) : 20(8): 2790-2813 Coenzyme Q0 inhibited the NLRP3 inflammasome, metastasis/EMT, and Warburg effect by suppressing hypoxia-induced HIF-1α expression in HNSCC cells
Pasula S et al. Front Genet 2022; 13 : 1011965 Systemic lupus erythematosus variants modulate the function of an enhancer upstream of <i>TNFAIP3</i>.
Jhih-Ying Chi et al. Clin Transl Med. 2022; 12 (11) : e1099 Blockade of the pentraxin 3/CD44 interaction attenuates lung injury-induced fibrosis
Application : WB
Reactivity : Human
Qiu W et al. Environ Sci Technol 2022; Antibiotic Chlortetracycline Causes Transgenerational Immunosuppression via NF-κB.
Bankole O et al. Int J Mol Sci 2022; 23 (3) Beneficial and Sexually Dimorphic Response to Combined HDAC Inhibitor Valproate and AMPK/SIRT1 Pathway Activator Resveratrol in the Treatment of ALS Mice.
Application : WB
Reactivity : Mouse
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Submit a Reference

REVIEW

NFkB p65 antibody Cat. No. GTX107678

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Date : Anonymous submitted on 23-Jun-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 10 μg
Blocking : 0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 12Hr
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-NFkB p65 antibody used in Western Blot (WB). GTX107678
Date : Anonymous submitted on 15-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 15 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
HL Yang et al. Int J Biol Sci 2024; 20 (8):20(8): 2790-2813 Coenzyme Q0 inhibited the NLRP3 inflammasome, metastasis/EMT, and Warburg effect by suppressing hypoxia-induced HIF-1α expression in HNSCC cells
Pasula S et al. Front Genet 2022; 13:1011965 Systemic lupus erythematosus variants modulate the function of an enhancer upstream of <i>TNFAIP3</i>.
Jhih-Ying Chi et al. Clin Transl Med. 2022; 12 (11):e1099 Blockade of the pentraxin 3/CD44 interaction attenuates lung injury-induced fibrosis
Application : WB
Reactivity : Human
Qiu W et al. Environ Sci Technol 2022; Antibiotic Chlortetracycline Causes Transgenerational Immunosuppression via NF-κB.
Bankole O et al. Int J Mol Sci 2022; 23 (3) Beneficial and Sexually Dimorphic Response to Combined HDAC Inhibitor Valproate and AMPK/SIRT1 Pathway Activator Resveratrol in the Treatment of ALS Mice.
Application : WB
Reactivity : Mouse
Xin Li et al. Transl Neurosci 2021; 12 (1):103-113 Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI).
Application : WB
Reactivity : Rat
Chu J et al. Evid Based Complement Alternat Med 2020; 2020:7905902 Huoxin Pill Attenuates Cardiac Inflammation by Suppression of TLR4/NF-κB in Acute Myocardial Ischemia Injury Rats.
Wu CS et al. Int J Immunopathol Pharmacol 2020; 34:2058738420929442 Phloretin alleviates dinitrochlorobenzene-induced dermatitis in BALB/c mice.
Shang S et al. Cancer Res 2019; (Epub) Macrophage ABHD5 suppresses NF-庥B-dependent matrix metalloproteinase expression and cancer metastasis.
Application : WB, ICC/IF
Reactivity : Mouse
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Schiaffino L et al. Sci Rep 2018; 8 (1):12875 Acetylation state of RelA modulated by epigenetic drugs prolongs survival and induces a neuroprotective effect on ALS murine model.
Application : IP
Reactivity : Mouse
Xiang W et al. Nat Commun 2018; 9 (1):2574 Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression.
Application : WB
Reactivity : Mouse
Chang TM et al. Evidence-Based Complementary and Alternative Medicine 2017; 2017:8325049 Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes.
Application : WB
Reactivity : Human
Chen CC et al. PLoS One 2016; 11 (12):e0168092 Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Application : WB, ICC/IF
Reactivity : Human
Chin YT et al. Mediators Inflamm 2016; 2016:6953459 2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis.
Application : WB
Reactivity : Human
Wang Y et al. Graefes Arch Clin Exp Ophthalmol 2016; Transplantation of IL-10-transfected endothelial progenitor cells improves retinal vascular repair via suppressing inflammation in diabetic rats.
Application : WB
Reactivity : Rat
Dhawan U et al. J Nanobiotechnology 2015; 13:79 Topological control of nitric oxide secretion by tantalum oxide nanodot arrays.
Application : WB
Reactivity : Rat
Raninga PV et al. Oncotarget 2015; (Epub) Inhibition of thioredoxin 1 leads to apoptosis in drug-resistant multiple myeloma.
Application : WB
Reactivity : Human
Chen T et al. Exp Dermatol 2014; Elevated serum HMGB1 levels in patients with Henoch-Schonlein purpura.
Application : ICC/IF
Reactivity : Human
Liu J et al. Arch Anim Nutr 2014; 68 (2):111-25 Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs.
Application : WB
Liang CJ et al. Evidence-Based Complementary and Alternative Medicine 2014; 2014 (305149):13 Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice
Application : WB
Reactivity : Human
Adrianto I et al. Nat Genet 2011; 43 (3):253-8 Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus.
Application : EMSA
Reactivity : Human
Liang CJ et al. Free Radic Biol Med 2011; 51 (7):1337-46 Viscolin reduces VCAM-1 expression in TNF-α-treated endothelial cells via the JNK/NF-κB and ROS pathway.
Application : WB
Reactivity : Human
Hsieh JY et al. Stem Cells Dev 2010; 19 (12):1895-910 Functional module analysis reveals differential osteogenic and stemness potentials in human mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord.
Application : WB
Reactivity : Human
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