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NFkB p65 antibody

Orthogonal
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX50371
Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50371
Anti-NFkB p65 antibody used in Western Blot (WB). GTX50371

Cat. No. GTX50371

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.274~278 (R-P-S-D-R) derived from human NFkB p65.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX50371

GTX50371 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50371 NFkB p65 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-NFkB p65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50371

GTX50371 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50371 NFkB p65 antibody.

Anti-NFkB p65 antibody used in Western Blot (WB). GTX50371

GTX50371 WB Image

Orthogonal

WB analysis of extracts from HeLa cells using GTX50371 NFkB p65 antibody with or without blocking peptide.

REFERENCE

Application Reference

Mahdi AH et al. Cancer Res 2018; (Epub) Evidence of inter-tissue differences in the DNA damage response and the pro-oncogenic role of NFκB in mice with disengaged BRCA1-PALB2 interaction.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399