GeneTex
United States (US)

NFkB p65 (phospho Ser311) antibody

GTX32255_20190131_IHC-P_19013101_188.jpg
GTX32255_20190131_ICCIF_19013101_941.jpg
GTX32255_20190131_WB_19013101_899.jpg

Cat No. GTX32255

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of NFkB p65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rela Proto-Oncogene, Nf-Kb Subunit,Nfkb3,P65,Rela

Cellular Localization

Cytoplasm,Nucleus

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
GTX32255_20190131_IHC-P_19013101_188.jpg

GTX32255 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32255 NFkB p65 (phospho Ser311) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32255_20190131_ICCIF_19013101_941.jpg

GTX32255 ICC/IF Image

ICC/IF analysis of formalin-fixed PC12 cells using GTX32255 NFkB p65 (phospho Ser311) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32255_20190131_WB_19013101_899.jpg

GTX32255 WB Image

WB analysis of TNFa-treated HeLa (A), mouse kidney (B), UV-treated PC12 (C) whole cell lysates using GTX32255 NFkB p65 (phospho Ser311) antibody.

Package List Price ($)
$ 319