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NFkB p65 (phospho Ser468) antibody

Anti-NFkB p65 (phospho Ser468) antibody used in Western Blot (WB). GTX54673

Cat No. GTX54673

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
Package
100μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic phosphorylated peptide around S468 of human RELA (NP_001138610.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rela Proto-Oncogene, Nf-Kb Subunit,Nfkb3,P65,Rela

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
Anti-NFkB p65 (phospho Ser468) antibody used in Western Blot (WB). GTX54673

GTX54673 WB Image

WB analysis of HeLa cells (20ng/ml treated with TNF-α for 30 minutes) using GTX54673 NFkB p65 (phospho Ser468) antibody.
Dilution : 1:2000
Loading : 25μg per lane

REFERENCE
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REVIEW
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Package List Price ($)
$ 319