WB analysis of Jurkat cells unstimulated (lane 1) or stimulated with PMA and Ca2+ (lane 2-5) using GTX17575 NFkB p65 (phospho Ser529) antibody.
Lane 1 and 2 : no peptide
Lane 3 : non-phospho peptide
Lane 4 : generic [pS] peptide
Lane 5 : phosphopeptide immunogen

IHC-P analysis of human spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody.
Right : Primary antibody
Left : a negative control without primary antibody
Dilution : 1:20 overnight at 4ºC
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins

IHC-P analysis of mouse spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody.
Right : Primary antibody
Left : a negative control without primary antibody
Dilution : 1:50 overnight at 4ºC
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins