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NFkB p65 (phospho Ser529) antibody

Orthogonal
Anti-NFkB p65 (phospho Ser529) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50254
Anti-NFkB p65 (phospho Ser529) antibody used in IHC (Paraffin sections) (IHC-P). GTX50254
Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX50254
Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX50254
Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX50254

Cat. No. GTX50254

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 529 (L-L-S(p)-G-D) derived from human NFkB p65.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11175285

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 (phospho Ser529) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50254

GTX50254 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50254 NFkB p65 (phospho Ser529) antibody.

Anti-NFkB p65 (phospho Ser529) antibody used in IHC (Paraffin sections) (IHC-P). GTX50254

GTX50254 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50254 NFkB p65 (phospho Ser529) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX50254

GTX50254 WB Image

WB analysis of extracts from 293 cells untreatedor treated with TNF-α using GTX50254 NFkB p65 (phospho Ser529) antibody.

Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX50254

GTX50254 WB Image

WB analysis of extracts from MCF cells treated with TNFa+CA or calf intestinal phosphatase (CIP) using GTX50254 NFkB p65 (phospho Ser529) antibody.

Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX50254

GTX50254 WB Image

Orthogonal

The data was published in the Antioxidants (Basel) in 2019. PMID: 31906147

REFERENCE

Application Reference

Manuela Leo et al. Antioxidants (Basel) 2022; 11 (4) Polyphenols Extracts from Oil Production Waste Products (OPWPs) Reduce Cell Viability and Exert Anti-Inflammatory Activity via PPARγ Induction in Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Kang-Hsi Wu et al. Int J Mol Sci 2022; 23 (10) Immunomodulation via MyD88-NFκB Signaling Pathway from Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury.
Application : WB, IHC-P
Reactivity : Mouse
Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy.
Application : WB
Reactivity : Human
Huang YJ et al. ACS Appl Mater Interfaces 2018; (Epub) Modulation of macrophage phenotype by biodegradable polyurethane nanoparticles: the possible relation between macrophage polarization and immune response of nanoparticles.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399