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NFkB p65 (phospho Ser536) antibody

Orthogonal
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899
Anti-NFkB p65 (phospho Ser536) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133899
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

Cat. No. GTX133899

Reference 9
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser536 of human NFkB p65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887135

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

GTX133899 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

GTX133899 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 (phospho Ser536) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133899

GTX133899 ICC/IF Image

NFkB p65 (phospho Ser536) antibody detects NFkB p65 (phospho Ser536) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFkB p65 (phospho Ser536) stained by NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

GTX133899 WB Image

Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35308231

REFERENCE

Application Reference

Li D et al. Heliyon 2024; 10 (5) : e27644 DL-3-n-butylphthalide attenuates doxorubicin-induced acute cardiotoxicity via Nrf2/HO-1 signaling pathway.
Li CJ et al. J Hepatocell Carcinoma 2023; 10 : 123-138
Application : WB
Reactivity : Human
Zhang W et al. Oxid Med Cell Longev 2023; 2023 : 9966355 Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
Application : WB
Reactivity : Mouse, Rat
Alhasan H et al. Nat Commun 2022; 13 (1) : 3919 Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis.
Application : WB
Reactivity : Human
Chou LY et al. Cells 2022; 11 (20) Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB Pathway.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

NFkB p65 (phospho Ser536) antibody Cat. No. GTX133899

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : ARPE19 cell lysates
Amount used : 10μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 16°C, 4Hr
Submit a Review
Application Reference
Li D et al. Heliyon 2024; 10 (5):e27644 DL-3-n-butylphthalide attenuates doxorubicin-induced acute cardiotoxicity via Nrf2/HO-1 signaling pathway.
Li CJ et al. J Hepatocell Carcinoma 2023; 10:123-138
Application : WB
Reactivity : Human
Zhang W et al. Oxid Med Cell Longev 2023; 2023:9966355 Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
Application : WB
Reactivity : Mouse, Rat
Li Y et al. Exp Ther Med 2022; 24 (3):567 Interleukin 32 participates in cardiomyocyte-induced oxidative stress, inflammation and apoptosis during hypoxia/reoxygenation via the NOD2/NOX2/MAPK signaling pathway.
Application : WB
Reactivity : Human
Alhasan H et al. Nat Commun 2022; 13 (1):3919 Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis.
Application : WB
Reactivity : Human
Chou LY et al. Cells 2022; 11 (20) Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB Pathway.
Application : WB
Reactivity : Human
Xue Zhang et al. Front Pharmacol 2022; 13:827128 A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.
Application : WB
Reactivity : Human
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169