GeneTex

  • Country / Location Selection

United States (US)

NFkB p65 (phospho Thr254) antibody

Anti-NFkB p65 (phospho Thr254) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50097
Anti-NFkB p65 (phospho Thr254) antibody used in IHC (Paraffin sections) (IHC-P). GTX50097
Anti-NFkB p65 (phospho Thr254) antibody used in Western Blot (WB). GTX50097

Cat. No. GTX50097

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from human NFkB p65.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11163335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 (phospho Thr254) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50097

GTX50097 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50097 NFkB p65 (phospho Thr254) antibody.

Anti-NFkB p65 (phospho Thr254) antibody used in IHC (Paraffin sections) (IHC-P). GTX50097

GTX50097 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50097 NFkB p65 (phospho Thr254) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-NFkB p65 (phospho Thr254) antibody used in Western Blot (WB). GTX50097

GTX50097 WB Image

WB analysis of extracts from 3T3 cells untreated or treated with TNF-a using GTX50097 NFkB p65 (phospho Thr254) antibody.

REFERENCE

Application Reference

Er-Chieh Cho et al. Mediators of Inflammation 2015; RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for NFkB p65 (phospho Thr254) antibody (GTX50097). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399