WB analysis of TNFa-treated Jurkat (A), TNFa-treated HepG2 (B) whole cell lysates using GTX54983 NFkB p65 (phospho Thr435) antibody.

ICC/IF analysis of formalin-fixed K562 cells using GTX54983 NFkB p65 (phospho Thr435) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX54983 NFkB p65 (phospho Thr435) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)